Introduction to the main uses of orcinol [Determination of ribose]_Kain Industrial Additives

OrcinolAlso known as lichenol, if the commercially available product has been stored It will be oxidized to red after a long time, and it needs to be recrystallized with hot benzene before use.

【Chemical Properties】
White rhombus crystal. The melting point is 58°C, and the anhydrous melting point is 107-108°C. Boiling point: 289-290℃, 165-170℃ (1.87-2.67kPa), 147℃ (0.67kPa). Relative density 1.290 (4/4℃). Easily soluble in water, alcohol and ether, slightly soluble in benzene, slightly soluble in chloroform and carbon disulfide. Easily oxidized in air and turns red. Has a sweet but unpleasant taste.

【Product Usage】
Analytical reagents for the determination of antimony, chromium, nitrate and nitrite, as well as pentose, lignin, sugar gum, aldose, beet sugar, sucrose and amylase. This product is irritating, and its oral LD50 in rats is 844 mg/kg.

Ribonucleic acid is one of two types of nucleic acids. The abbreviation symbol is RNA. It is a biological macromolecule mainly composed of four kinds of ribonucleotides connected in a certain order by 3′, 5′-phosphodiester bonds. Slightly soluble in water, insoluble in ethanol; under alkaline conditions, hydrolyzed to generate 2′- or 3′-nucleotide; has obvious absorption of 260 nm ultraviolet light; and 3,5-dihydroxytoluene (also known as lichenol Or orcinol) reagent reacts to produce a green substance. It exists in some viruses and all biological cells, and its main function is to participate in protein biosynthesis. According to function, it is divided into transfer ribonucleic acid (tRNA), messenger ribonucleic acid (mRNA), ribosomal ribonucleic acid (rRNA) and other ribonucleic acids with specific functions. Yeast contains a lot of RNA and is often used as a preparation material.

【Oakmoss Extract】
A paste-like spice. It has a refreshing moss aroma, like musk and lavender, and the fragrance lasts a long time. The product extracted with benzene is a brown-black solid wax with a melting point of 51°C, an acid value of 16.1 to 22.4, and an ester value of 38 to 24. The product extracted with petroleum ether is a reddish-brown semi-solid with a poor aroma, a melting point of 48-50°C, an acid value of 56.8-68.2, and an ester value of 74.2-78.5°C. The product extracted with ethanol is semi-liquid with an acid value of 32.6-72.3 , with an ester value of 62.9~82.7°C. Pure oil can be obtained by leaching the extract, and colorless concentrated essential oil can be obtained by distilling the extract under reduced pressure. The main components of the extract include orcinol monomethyl ether, terpenes, and coal lichen Methyl and ethyl esters, aldehydes, ketones, etc. are obtained from oakmoss plants. Produced in France, Czech Republic, Italy, Hungary, South Africa, Morocco and other countries, and also in Yunnan and Shaanxi in China. Its extract can be made from benzene, ethanol, Extracted from petroleum ether. It is a good fixative. Used in the preparation of perfumes, cosmetics and soap flavors.

【Measurement of Ribose】
1. Ribose is dehydrated and cyclized in concentrated acid to form furfural, which reacts with orcinol (3,5-dihydroxytoluene) to show blue-green color and has a maximum absorption value at 670nm.
2. Reagents
Orcinol reagent: Combine orcinol (also known as lichenol, if it is a commercially available product that is oxidized to red, it needs to be recrystallized with hot benzene before use), ferric ammonium sulfate (ferric chloride can also be used instead) 13.5g, make 500mL with distilled water and store at low temperature. Before use, take 2.5mL of the stock solution, add 41.5mL of 12mol/L hydrochloric acid, and add distilled water to 50mL, which is the orcinol application solution.
3. Measurement
Take 1 mL of sample solution, add 3 mL of orcinol application solution, heat in a boiling water bath for 20 minutes, immediately cool with cold water, zero with reagent blank, measure the A670nm value, compare with the standard sample, and calculate the ribose content in the sample.
4. Description
Proteins and mucopolysaccharides may interfere with the determination.

The above information was edited and compiled by Andy from ChemicalBook.